Biochem/physiol Actions

Reversible: yes

Product competes with ATP.

Primary TargetPKC

Target IC₅₀: 7.9 nM, 2.3 nM, and 6.2 nM, for rat brain PKC, Ca2+-dependent PKC α-isozyme, and PKCβI, respectively

Cell permeable: yes

General description

Inhibitor of protein kinase C (PKC; IC₅₀ = 7.9 nM for rat brain). Selectively inhibits Ca²⁺-dependent PKC_α-isozyme (IC₅₀ = 2.3 nM) and PKC_βI (IC₅₀ = 6.2 nM). Does not affect the kinase activity of the Ca²⁺-independent PKC δ-, ε-, and ζ-isoenzymes even at micromolar levels. A potent antagonist of HIV-1 induction.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Gschwendt, M., et al. 1996. FEBS Lett.392, 77.Wenzel-Seifert, K., et al. 1994. Biochem. Biophys. Res. Commun.200, 1536.Martiny-Baron, G., et al. 1993. J. Biol. Chem.268, 9194.Qatsha, K.A., et al. 1993. Proc. Natl. Acad. Sci. USA90, 674.

Packaging

Packaged under inert gas

1 ml in Plastic ampoule

Physical form

A 500 µg/ml solution of Gö 6976 (catNO=365250">Cat. No. 365250) in anhydrous DMSO.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Irritant (B)